Methoden

Zellbiologie

Zellkultur (mammalian (primäre Zellen, Zelllinien), Hefe, E. coli), Transfektion (Nucleofection), Blutzellisolierung (Ficoll), histologische Färbungen, Immunzytochemie, Immunhistochemie

(Protein)-Biochemie

SDS-PAGE, Western Blot, 2D-Gelelektrophorese (Blaue-Native-/SDS-Tricin-PAGE, IEF/SDS-PAGE), 2D-DIGE Analyse, rekombinante Protein Expression, Proteinaufreinigung, pull down Assays, (Co)-Immunpräzipitation, Isolierung nativer Membran-gebundener Protein-Komplexe, Größenausschlusschromatographie (SMART-FPLC), Yeast-2-Hybrid, verschiedene Gradienten Techniken (Sedimentation, Flotation, Dichte)

Molekularbiologie

PCR, Klonierungs-Techniken, site-directed Mutagenesis, RNA/DNA Extraktion und Präparation, cDNA Synthese, Primerdesign, quantitative Real-Time PCR (Sybr Green)

Originalartikel

Rosenkranz, K, Kumbruch, S, Lebermann, K, Marschner, K, Jensen, A, Dermietzel, R, and Meier, C, The chemokine SDF-1 / CXCL12 contribute to the ‘homing’ of umbilical cord blood cells to a hypoxic-ischemic lesion in the rat brain, J Neurosci Res 2009; in press

Rosenkranz, K*, Birschmann, I*, Grunau, S, Girzalsky, W, Kunau, W-H, and Erdmann, R, Functional association of the AAA-complex and the peroxisomal importomer, FEBS J. 2006 Aug; 273(16): 3804-15.
*both authors contributed equally to this manuscript

Halbach, A, Landgraf, C, Lorenzen, S, Rosenkranz, K, Volkmer-Engert, R, Erdmann, R, and
Rottensteiner, H, Targeting of the tail-anchored peroxisomal membrane proteins PEX26 and PEX15 occurs via C-terminal PEX19-binding sites, J Cell Sci. 2006 Jun 15;119(Pt 12): 2508-17.

Platta, HW, Grunau, S, Rosenkranz, K, Girzalsky, W, and Erdmann, R, Functional role of the AAA peroxins in dislocation of the cycling PTS1 receptor back to the cytosol, Nat Cell Biol. 2005 Aug;7(8): 817-22.

Birschmann, I*, Rosenkranz, K*, Erdmann, R., and Kunau, WH , Structural and functional analysis of the interaction of the AAA-peroxins Pex1p and Pex6p, FEBS J. 2005 Jan; 272(1): 47-58.
*both authors contributed equally to this manuscript

Birschmann, I, Stroobants, AK, van den Berg, M, Schaefer, A, Rosenkranz, K, and Kunau, WH
Pex15p of Saccharomyces cerevisiae provides a molecular basis for recruitment of the AAA peroxin Pex6p to peroxisomal membranes, Mol Biol Cell. 2003 Jun; 14(6): 2226-36.